Fast and Efficient Single-stranded Genomic Library ...
Key steps of the 3 library preparation methods compared here. (A) The BEST protocol begins with an input DNA end-repair step where 3′ overhangs are digested and 5′ overhangs are filled in (1).Then, double-stranded adapters are ligated to the 5′ ends of the input DNA (2), followed by adapter fill-in with a polymerase extension step, which initiates at the 3′ …